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Title:
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An ensemble of new techniques to study soft-X-ray-induced variations in cellular metabolism
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Permalink:
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http://hdl.handle.net/10446/20230
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| Suggested citation: | PREVIDI, FABIO, AA.VV., (2004). An ensemble of new techniques to study soft-X-ray-induced variations in cellular metabolism. Laser and particle beams (3/22), 323- 333. Retrieved from http://hdl.handle.net/10446/20230 |
| Author⁄s: | PREVIDI, FABIO
AA.VV. |
| Type of content: | journal article - articolo |
| Language: | eng |
| Date issued: | 2004 |
| Is part of: | Laser and particle beams |
| Issue: | 3/22 |
| From p. : | 323 |
| to p. : | 333 |
| Keywords (Italian): | Laser plasmas;Metabolism;Radiobiology;Soft X-rays;X-ray dosimetry |
| Abstract: | An ensemble of new techniques has been developed to study cell metabolism. These include: CO2 productionmonitoring, cell irradiation with soft X rays produced with a laser-plasma source, and study of oscillations in cellmetabolic activity via spectral analysis of experimental records. Soft X-rays at about 0.9 keV, with a very low penetration in biological material, were chosen to produce damages at the metabolic level, without great interference with DNA activity. The use of a laser-plasma source allowed a fast deposition of high doses. Monitoring of CO2 production allowed us to measure cell metabolic response immediately after irradiation in a continuous and noninvasive way. Also a simple model was developed to calculate X-ray doses delivered to the different cell compartments following a Lambert–Bouguet–Beer law. Results obtained on Saccharomyces cerevisiae yeast cells in experiments performed at Rutherford Appleton Laboratory are presented |
| Other info: | Questo articolo propone un nuovo metodo per lo studio delle modificazioni del metabolismo cellulare indotte da interazioni con radiazioni non ionizzanti. Tale metodo è basato sull’uso di raggi X molli che non penetrano fino al nucleo cellulare (danneggiando il DNA), ma limitano la loro azione agli strati periferici (membrana e citoplasma) che sono la sede dei meccanismi metabolici. Lo studio è corredato da numerose prove sperimentali.
impact factor 0.575 |
| In: | Journal contributions - Contributi su rivista scientifica
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