An ensemble of new techniques has been developed to study cell metabolism. These include: CO2 production monitoring, cell irradiation with soft X rays produced with a laser-plasma source, and study of oscillations in cell metabolic activity via spectral analysis of experimental records. Soft X-rays at about 0.9 keV, with a very low penetration in biological material, were chosen to produce damages at the metabolic level, without great interference with DNA activity. The use of a laser-plasma source allowed a fast deposition of high doses. Monitoring of CO2 production allowed us to measure cell metabolic response immediately after irradiation in a continuous and non invasive way. Also a simple model was developed to calculate X-ray doses delivered to the different cell compartments following a Lambert-Bouguet–Beer law. Results obtained on Saccharomyces cerevisiae yeast cells in experiments performed at Rutherford Appleton Laboratory are presented.

An ensemble of new techniques to study soft-X-ray-induced variations in cellular metabolism

PREVIDI, Fabio;
2004-01-01

Abstract

An ensemble of new techniques has been developed to study cell metabolism. These include: CO2 production monitoring, cell irradiation with soft X rays produced with a laser-plasma source, and study of oscillations in cell metabolic activity via spectral analysis of experimental records. Soft X-rays at about 0.9 keV, with a very low penetration in biological material, were chosen to produce damages at the metabolic level, without great interference with DNA activity. The use of a laser-plasma source allowed a fast deposition of high doses. Monitoring of CO2 production allowed us to measure cell metabolic response immediately after irradiation in a continuous and non invasive way. Also a simple model was developed to calculate X-ray doses delivered to the different cell compartments following a Lambert-Bouguet–Beer law. Results obtained on Saccharomyces cerevisiae yeast cells in experiments performed at Rutherford Appleton Laboratory are presented.
journal article - articolo
2004
Turcu, Edmond; Allot, Rick; Lisi, Nicola; Batani, Dimitri; Bortolotto, Fulvia; Masini, Alessandra; Milani, Marziale; Ballerini, Monica; Ferraro, Lorenzo; Pozzi, Achille; Previdi, Fabio; Rebonato, Lorenzo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10446/20230
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